Extracellular vesicles (EVs) are key players in biological and medical research, yet their efficient purification from body fluids like plasma and serum remains a significant challenge. Their low concentration, compared to the abundant lipoproteins (with similar size and density), complicates the purification process. Lipoproteins make up ~99.9998% of blood particles, leaving EVs at a mere ~0.0002% (source: https://lnkd.in/grBDnqkc).

🔬 Our Breakthrough Solution: PEMD for Immunoaffinity-Based Purification
Acytronix has developed a groundbreaking method to selectively purify EVs from plasma and serum using immunoaffinity. By targeting unique protein markers like CD9, our approach allows for ultra-pure EV purification in under 10 minutes.

Key Advantages of Our Technology:
✅ Efficient Capture: Achieves theoretical saturation levels of ~3×10¹⁰ EVs per mL of plasma (ISEV paper).
✅ Selective Purification: Free from contamination by lipoproteins or other soluble proteins.
✅ High Functional Concentration: Perfect for proteomics and biological analysis, ensuring maximal EV activity.

🔬Proven Results:
Our method drastically increases the concentration of EV markers like CD9 and TSG101 compared to other methods. See the attached Western Blot analysis for clear evidence of enhanced EV purification with PMED.

🔬Clinical Applications:
The attached illustration shows clear EV purification between healthy and unhealthy mice, paving the way for advanced proteomic analysis and providing critical insights into health conditions. This research, which demonstrates our technology, was published as a cover article in the prestigious Advanced Materials journal (source: https://lnkd.in/eDi6zWVr).

🌟 Acytronix is leading the way. Whether you’re in EV research or developing EV-based diagnostics, join us in revolutionizing this space!